Product Introduction广东品博易视生物科技有限公司

PinpoRNA probe set

PinpoRNA probes are available in a variety of types, such as Type I, II, III, IV and V, which are distinguished by the last digit of the catalog number (1/2/3/4/5). Different types of probes can be used individually or mixed with other types for multiplex co-staining in the same section. However, in principle, probes of the same type cannot be used at the same time, because they will be dyed with the same color and cannot be distinguished. The difference between the different types of probes is that the recognition sequences attached to the primers can be recognized by different amplification systems. The catalog number of a PinpoRNA probe typically consists of the following parts (#####N-&T). "#####" is the generic gene ID of the target gene. "N" is a number, and different numbers represent different detection sequences covered. "&" is the letter for species information, A for human (Homo sapiens), B for mouse (Mus musculus), C for rat (Rattus norvegicus), D for other species. "T" is the number 1/2/3/4/5, which represents the type of probe. You may search in “catalog probe” to retrieve probe specific information. The probes are shipped in two packages, the first is a ready-to-use version that can be used dropwise (heat to dissolve the precipitate and mix well before use). The second is the concentrated version (typically 40x), which does not require heat but must be diluted with a ready-to-use probe and mixed for multicolor detection, or diluted with a probe dilution (PGR1007) without any probes for single-color detection.

Manual detection kits

The manual detection kit is suitable for low-throughput detection in ordinary laboratories, which is economical and practical to save reagents, and is suitable for optimizing some experimental conditions. Manual testing must also be used to verify the assay result before using automated instruments for high-throughput testing.

Automation kits

The automation assay kits can be used with a variety of automatic in-situ hybridizers to achieve high-throughput rapid detection, please consult technical support for details.

fluorescent assay kit

The fluorescence assay kit uses tyramide signal amplification (TSA) technology to label the gene of interest. The higher resolution of the detection results allows for the spatial localization of a single copy. The labeling order and fluorescence channel can be freely selected for each target gene to achieve the best picture effect. However, the interpretation of fluorescence kit results is susceptible to the autofluorescence of the sample. Single-color fluorescent kits can label a gene of interest, and the localization and quantification results are very accurate. Multicolor fluorescent kits can label multiple genes, but their localization quantification results are affected by the effect of spatial holdings issue. The labelling sequence of multiple markers may affect the interpretation of localization results.

Chromogenic assay kit

Compared with the fluorescence assay kit, the chromogenic kit has higher sensitivity on tissue sections, which can be observed by ordinary light microscopes, and is not interfered by the autofluorescence of tissues. The results are clearer and more intuitive, and the staining results can be reserved for a long time. It is suitable for qualitative and semi-quantitative detection of large batches of samples. The disadvantage is that, the most only two target genes can be detected on one slide, and the spatial holding effect is more serious than that of fluorescence detection in dual-gene detection. And the target protein must also be chromogenic stained when co-staining with proteins. Single-color red chromogenic kit, using Fast Red substrate, the signal contrast is stronger and easier to observe. Single-color brown chromogenic kit using DAB substrate, the signal suitable for long-term storage. The two-color chemical chromogenic kit (red-brown, red-green) is suitable for replacing multicolor fluorescence experiments on samples with a high auto-fluorescence background, such as the human brain.

adjuvant pathology testing kits

We have developed and validated a serial of potential diagnostic targets that can be used in companion diagnostics. The assay protocol are identical to PinpoRNA chromogenic or fluorescent assay kits. The kit packaging size is suitable for automation instruments. According to customer requirements, the test target or packaging volume can be customized and developed, or commissioned production. Please contact us for details.

Assay kit only for cell type

The detection technology of cell-specific detection reagents is to use fluorescent molecules for direct labeling, so there are fewer experimental steps and faster to get results (within 3 hours). The sensitivity can reach 1 copy/cell, which can be localized to the subcellular level and accurately quantified. The multiplex detection of up to 4 targets can be performed simultaneously. It is suitable for normal cultured cells in multi-well plates (96/384 wells) for high-throughput testing. It is suitable for high-content scanner to do photo capture and analysis, especially important for drug R&D testing of CRO/pharmaceutical and other enterprises.